Primer Probe Design Tool
Number of Primer/Probe Sets to Return (Limit 20 Sets): PCR Amplicon Size Range: Primer Tm: Minimum Optimum Maximum °C Probe Tm: Minimum Optimum Maximum °C Organism: Pick Primer/Probe Crossing Exon Junction When Pick Primer/Probe Crossing Exon Junction is selected, the exon regions must be defined. If only raw sequence is provided, the sequence will be mapped on the genome (human, mouse or rat at present) sequences to locate the exon boundaries. Besides, there are other two ways to specify exon regions: • The best way is to fill in GenBank Accession with the accession number ( e.g. NM_145027, please note the accession number is case-sensitive and should not contain version suffix like '.1' or '.2'). The sequence will be fetched from NCBI data center, and the exon regions are already defined in sequence annotations.
• Manually specify the exon junctions with ':' in the input sequence (e.g. 'ACGCGCG:CGTACG') Target Nucleotide Sequences: GenBank Accession: or Paste in the DNA Sequence in raw format. Rolemaster rulebook pdf.
Primer Design Tool Pdf
The easy-to-use ProbeFinder Assay Design Software displays the target-specific primer sequences (ready for ordering at your preferred oligo supplier) and the matching Universal ProbeLibrary probe. Universal ProbeLibrary assays are compatible with all real-time PCR instruments capable of detecting fluorescein, FITC, FAM, and/or SYBR ® Green I.
Design your own Custom Stellaris RNA FISH probes using our online Stellaris probe designer, free with registration. By using this probe designer, you are just a few clicks away from placing your Stellaris order online.
LGC Biosearch Technologies' Stellaris ® RNA FISH Probe Designer, for RNA in situ hybridization probe design, is easy to use and free of charge with Biosearchtech.com registration. You may use the designer for in situ probe design, review your sequences, save them to your cart, and proceed to online checkout with just a few clicks.